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 Sterilray Test Results
 

Founding Research

Sterilray™ was tested with and without water to determine its effect on organisms. The organism used in all tests was the MS-2 virus, which has become a standard indicator of mutation effectiveness. The EPA report (811-R-96-002) reports a 4.3 average log reduction of the MS-2 virus using mercury light at irradiance greater than 128 mj /cm2.

Three wavelengths were tested; Sterilray™, mercury(UVC at 254nm) and 259 nm. Sterilray™ was tested at three levels of irradiance with the virus in a thin layer of water in order to reduce the absorption effect of water. A separate test was also done with the virus in water. The mercury vapor(UVC) and 259 nm lamps were tested at the identical irradiance levels with the virus in water. Controls were made on all tests and a single test dish on each lamp was made to check experimental error.

Sterilray™ produced log 5 reductions at 40 mj/ cm2 and log 6.5 reductions at 60 mj/ cm2. The water test produced a 3.2 log reduction, which matched the equivalent calculated irradiance in air. The mercury and 259 nm lamps produced about log 4 reductions at 60 mj/cm2. A 3 million reduction in population is about 10 to 100 times more effective than reported mercury vapor results at the same irradiance.

The results of the test indicate that Sterilray™ is very effective in causing mutations in airborne organisms. These tests indicate an improvement of between 10 to 1000 times, depending on the intensity of the lamp. It is important to note the improvement of the 259 nm source compared to the mercury source. This produced a 10 times improvement in the test sample even in water. It illustrates the importance in using a UV photon emitter that is near the absorption peak of the DNA or targeted chemical such as proteins, nucleic acids, or amino-acids.

DNA Absorption:

It is important to note that DNA biochemical's will have different absorption spectra and the peak absorption will be shifted by water, ph, temperature, previously absorbed light and surrounding contaminates in the air. The presence of ozone can significantly reduce the resistance to damage and shorten the action kill time. Sterilray™ produces a small amount of ozone (less than 0.5 ppm) further improving its ability to kill DNA.

The energy of the emitted photon is determined by its wavelength. Photon energy is about 5ev at 250nm, and increases for shorter wavelengths. Different bonds in the DNA will be affected with photons of different energy.

The Sterilray™ photon has more energy and is absorbed by S-N, S-O, O-O, O-H, and many carbon bonds that do not absorb mercury light. Initial tests suggest that Sterilray™ prevents DNA repair that has been reported for low level mercury UV sources.

Chemical cleaners require from 30 seconds to 5 minutes of contact time to be effective in killing viruses. Still, their effectiveness is reported at about 87%, very low compared to Sterilray™.

 

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